Publications

Research Articles

Small Molecule Mass Spectrometry

Untargeted Metabolomics

Metabologenomics and Natural Products Drug Discovery

1. Bioactivity-driven fungal metabologenomics identifies antiproliferative stemphone analogs and their biosynthetic gene cluster. Metabolomics 2024, 20, 90.

2. Correlative metabologenomics of 110 fungi reveals metabolite-gene cluster pairs. Nature Chemical Biology 2023, 1-15.

Targeted Metabolomics

Amino Acids and Peptides

3. LC-MS/MS based separation and quantification of Marfey’s reagent derivatized proteinogenic amino acid dl-stereoisomers. Journal of American Society of Mass Spectrometry 2019, 30, 448-458.

4. Antibiotic effects on Staphylococcus aureus cytoplasmic cell wall intermediate levels and evidence for potential metabolite level regulatory loops. Antimicrobial Agents and Chemotherapy 2017, 61, e02253-16.

5. Cytoplasmic peptidoglycan intermediate levels in Staphylococcus aureus. Biochimie 2016, 121, 72-78.

6. Gaussian and linear deconvolution of LC-MS/MS chromatograms of the eight aminobutyric acid isomers. Analytical Biochemistry 2017, 516, 75-85.

Enzyme Activity Analysis

DNA Nucleosides

7. Efficient purification and LC-MS/MS-based assay development for ten-eleven translocation-2 5-methylcytosine dioxygenase, Journal of Visualized Expression 2018, 140, e57798.

8. Positive/negative ion-switching based LC-MS/MS method for quantification of cytosine derivatives produced by TET family 5-methylcytosine dioxygenases. Biology Methods and Protocols 2020, 5, 1-9.

High-Throughput Screening and Antibacterial Drug Discovery

Drug and Drug Metabolites

9. Dimensionally enhanced antibacterial library screening. ACS Chemical Biology 2019, 14, 2887-2894.

10. Qualification of Human Liver Microsomes for Antibacterial Activity Screening of Drug Metabolites. Applied Microbiology 2023, 3, 104-118.

11. Screening the NCI diversity set V for anti-MRSA activity: cefoxitin synergy and LC-MS/MS confirmation of folate/thymidine biosynthesis inhibition. Microbiology Spectrum 2023, e0054123.

Drug Formulation Characterization

Microparticle and Nanomicellar Formulations

12. Layer-by-layer engineering of HIV-1 gp120 responsive nanomicrobicide: physicochemical and biological responses. Molecular Pharmaceutics 2017, 14, 3512-3527.

13. Self-Assembled Nanomicelles to Enhance Solubility and Anticancer Activity of Etoposide, ASSAY and Drug Development Technologies, 2021, 19, 526-538.

14. Self-Assembled Nanomicellar Formulation of Docetaxel as a Potential Breast Cancer Chemotherapeutic System. Life, 2022, 12, 485.

Solid Dispersion and Salt Formulation

15. Engineering fast dissolving sodium acetate mediated crystalline solid dispersion of docetaxel. International Journal of Pharmaceutics 2018, 545, 329-341.

16. Enhancing Etoposide Aqueous Solubility and Anticancer Activity with L-Arginine. ASSAY and Drug Development Technologies, 2021, 19, 508-525.

Microspheres

17. Preparation and characterization of Gliclazide incorporated cellulosic microspheres: studies on drug release, compatibility and micromeritics. Dhaka University Journal of Pharmaceutical Science 2014, 13, 149-166.

18. Evaluation and optimization of influence of permeability property and concentration of polymethacrylic polymers on microspheres of Metformin HCl. Dhaka University Journal of Pharmaceutical Society 2013, 12, 131-141.

19. Preparation, characterization and compatibility studies of Naproxen loaded microspheres of cellulosic and polymethacrylic polymeric blend. Dhaka University Journal of Pharmaceutical Society 2013, 12, 11-21.

Protein Mass Spectrometry

Top-Down Proteomics

Native I2MS

20. Characterizing amyloid beta oligomers of differing origin by Individual Ion Mass Spectrometry. (Under Preparation).

Review Articles

1. High-Throughput Screening of Natural Product and Synthetic Molecule Libraries for Antibacterial Drug Discovery. Metabolites 2023, 13, 625.

2. Features, roles and chiral analysis of proteinogenic amino acids. AIMS Molecular Sciences 2020, 7, 229-268.

Posters

1. Jooß, K., Ives, A., Papin, A., Soshnev, A. A., Ayon, N. J. et al. (2023) Characterization of linker histone H1 proteoforms in naïve vs. germinal center B cells using CZE-MS. International Symposium on Microscale Separation and Bioanalysis.

2. Knox, B., Butun, F. A., Robey, M. T., Ayon, N. J. et al. (2023) Expanding functional access to fungal natural products with metabologenomics and heterologous expression. International Conference on Natural Product Discovery and Development in the Genomic Era.

3. Ayon, N. J., Caesar, L. K., Butun, F. A., et al. (2022) Identification of bioactive fungal secondary metabolites and their biosynthetic gene clusters using metabologenomics. American Society of Mass Spectrometry Conference.

4. Caesar, L. K., Butun, F. A., Ayon, N. J., et al. (2021) First Demonstration of combined Metabolomics and Genomics to Discover Bioactive Natural Products and their Biosynthetic Machinery in Fungi. American Society of Mass Spectrometry Conference.

5. Caesar, L. K., Butun, F. A., Ayon, N. J. et al. (2021) Integration of Metabolomic and Genomic Approaches to Explore Uncharted Biochemical Space in Fungi. American Council for Medicinally Active Plants Conference.

6. Ayon, N. J. et al. (2020) Multidimensional Chemical Library Screening for Novel Antibacterial Activities. American Association of Pharmaceutical Scientists Annual Conference.

7. Gargvanshi, S., Ayon, N. J. et al. (2020) Screening of FDA-Approved Drug Library against Vancomycin-resistant Enterococci. American Association of Pharmaceutical Scientists Annual Conference.

8. Sharma, A. D., Ayon, N. J. et al. (2019) Cell wall metabolite levels in S. aureus as a function of growth phase. American Association of Pharmaceutical Scientists Annual Conference.

9. Ayon, N. J. et al. (2019) Metabolism enhanced multiplexed FDA-approved drug screening for novel antibacterial activities. American Chemical Society National Meeting and Exposition.

10. Ayon, N. J., and Sobansky, M. R. (2019) Development and Optimization of Untargeted, Label Free, Bottom-Up Proteomics Workflow Using Ion Mobility Enabled Q-TOF LC-MS/MS for Plasma Protein Analysis. Streck Annual Intern Showcase.

11. Ayon, N. J. et al. (2019) LC-MS/MS based separation and quantification of Marfey’s derivatized Proteinogenic Amino Acid DL-stereoisomers. American Society of Mass Spectrometry Conference.

12. Ayon, N. J. et al. (2019) Serendipitous discovery of synergistic and antagonistic activity of FDA approved drugs against MRSA. UMKC Community of Scholar Symposium/Health Sciences Research Summit.

13. Ayon, N. J. et al. (2018) Discovery of antibacterial properties of pyrimidine derivatives by high throughput screening of pre-metabolized chemical library. American Association of Pharmaceutical Scientists Conference.

14. Ayon, N. J. et al. (2018) LC-MS/MS-based analytical method for Marfey's reagent derivatized amino acid stereoisomers. American Chemical Society National Meeting and Exposition.

15. Ayon, N. J. et al. (2018) Using biological chemistry for novel antibacterial agent discovery by high throughput screening of pre-metabolized chemical library. UMKC Community of Scholar Symposium.

16. Ayon, N. J. et al. (2017) Enhanced chemical diversity through library in situ pre-metabolism: Using biological chemistry for novel lead discovery. American Chemical Society National Meeting and Exposition.

17. Ayon, N. J. et al. (2016) Analytical method development for the determination of D-and L-amino acids by high performance liquid chromatography tandem mass spectrometry. Pharmaceutics Graduate Student Regional Meeting.

18. Vemula, H., Ayon, N. J. et al. (2016) Profiling of bacterial cell wall peptidoglycan pathway inhibitors using LC-MS/MS-based quantification of cytoplasmic pathway intermediates. American Chemical Society National Meeting and Exposition.

Presentations

1. McGee, J. P., Ayon, N. J., Kandi, S. et al. (2022) Probing Amyloid Beta Oligomers from Alzheimer’s Disease with Ion Mobility and Tandem Individual Ion Mass Spectrometry. American Society of Mass Spectrometry Conference.

2. Ayon, N. J., Kelleher, N. et al. (2021) Scaling multi-omics to uncover microbial natural products for enhanced sustainability. American Chemical Society National Meeting and Exposition.

3. Ayon, N. J. (2020) Dimensionally enhanced antibacterial library screening and application of mass spectrometry in DPPS research and beyond. Spring 2020 Seminar Series, UMKC School of Pharmacy, Kansas City, MO, USA.

4. Ayon, N.J. (2019) Development and optimization of proteomics workflow for plasma protein analysis. Streck’s Annual Intern Showcase Event, La Vista, NE, USA.

5. Ayon, N. J. (2019) Antibacterial drug repurposing using multiplexed compound library screening. Community of Scholars, Kansas City, MO, USA.

6. Ayon, N. J. (2018) Enhanced chemical diversity through library in-situ pre-metabolism: using biological chemistry for novel lead discovery. Spring 2018 Seminar Series, UMKC School of Pharmacy, Kansas City, MO, USA.

7. Ayon, N. J. (2016) Analytical method development for the determination of d- and l-amino acids by high performance liquid chromatography tandem mass spectrometry. Fall 2016 Seminar Series, UMKC School of Pharmacy, Kansas City, MO, USA.

Three Minute Thesis

1. Ayon, N. J. (2019) Separation of amino acid mirror images. Community of Scholars, Kansas City, MO, USA.

2. Ayon, N. J. (2018) Does biochemical transformation increase antibacterial hits? Community of Scholars, UMKC, Kansas City, MO, USA.

Metabolomics and Chemical Library Screening for Antibacterial Drug Discovery